Instead of doing global alignment on whole genome sequences, align just the searched subtype ORF sequence to the full query sequence. The searched subtype ORF sequence should be prefixed and suffixed with "+" to discourage spread of nucleotides.
Instead of doing global alignment on whole genome sequences, align just the searched subtype ORF sequence to the full query sequence.
The searched subtype ORF sequence should be prefixed and suffixed with "+" to discourage spread of nucleotides.