diff --git a/pyathena/arrow/result_set.py b/pyathena/arrow/result_set.py
index ea11a419f..799bbfb97 100644
--- a/pyathena/arrow/result_set.py
+++ b/pyathena/arrow/result_set.py
@@ -308,7 +308,8 @@ def _read_csv(self) -> Table:
             parse_opts = csv.ParseOptions(
                 delimiter=",",
                 quote_char='"',
-                ignore_empty_lines=not binary_columns,
+                # Athena writes a single-column row with a NULL value as an empty line.
+                ignore_empty_lines=False,
                 double_quote=True,
                 escape_char=False,
             )
diff --git a/tests/pyathena/arrow/test_cursor.py b/tests/pyathena/arrow/test_cursor.py
index af1abca69..8d8f66bd6 100644
--- a/tests/pyathena/arrow/test_cursor.py
+++ b/tests/pyathena/arrow/test_cursor.py
@@ -46,6 +46,22 @@ def test_binary_single_null(self, arrow_cursor):
         arrow_cursor.execute("SELECT CAST(NULL AS VARBINARY) AS value")
         assert arrow_cursor.fetchall() == [(None,)]
 
+    @pytest.mark.parametrize(
+        ("query", "expected"),
+        [
+            (
+                "SELECT x FROM (VALUES 1, NULL, 2) AS t(x) ORDER BY x NULLS FIRST",
+                [(None,), (1,), (2,)],
+            ),
+            # Arrow reads a NULL string from a CSV result as an empty string.
+            ("SELECT CAST(NULL AS VARCHAR) AS v", [("",)]),
+        ],
+    )
+    def test_single_column_null(self, arrow_cursor, query, expected):
+        arrow_cursor.execute(query)
+        assert arrow_cursor.as_arrow().num_rows == len(expected)
+        assert arrow_cursor.fetchall() == expected
+
     @pytest.mark.parametrize(
         "arrow_cursor",
         [{"cursor_kwargs": {"unload": False}}, {"cursor_kwargs": {"unload": True}}],