diff --git a/folium/utilities.py b/folium/utilities.py
index d6f40580b1..e9807ad91d 100644
--- a/folium/utilities.py
+++ b/folium/utilities.py
@@ -122,6 +122,8 @@ def _validate_locations_basics(locations: TypeMultiLine) -> None:
 def validate_locations(locations: TypeLine) -> list[list[float]]:
     """Validate an iterable with lat/lon coordinate pairs."""
     locations = if_pandas_df_convert_to_numpy(locations)
+    if isinstance(locations, Iterator):
+        locations = list(locations)
     _validate_locations_basics(locations)
     return [validate_location(coord_pair) for coord_pair in locations]
 
@@ -131,7 +133,12 @@ def validate_multi_locations(
 ) -> Union[list[list[float]], list[list[list[float]]]]:
     """Validate an iterable with possibly nested lists of coordinate pairs."""
     locations = if_pandas_df_convert_to_numpy(locations)
+    if isinstance(locations, Iterator):
+        locations = list(locations)
     _validate_locations_basics(locations)
+    # Keep the nesting check below from consuming a point from an inner iterator.
+    if isinstance(next(iter(locations)), Iterator):
+        locations = [list(line) for line in locations]
     try:
         float(next(iter(next(iter(next(iter(locations)))))))  # type: ignore
     except (TypeError, StopIteration):
diff --git a/tests/test_utilities.py b/tests/test_utilities.py
index a1f10be361..26d346ca39 100644
--- a/tests/test_utilities.py
+++ b/tests/test_utilities.py
@@ -80,6 +80,20 @@ def test_validate_locations(locations):
     assert outcome == [[0.0, 5.0], [1.0, 6.0], [2.0, 7.0]]
 
 
+@pytest.mark.parametrize("validator", [validate_locations, validate_multi_locations])
+@pytest.mark.parametrize("count", [1, 3])
+@pytest.mark.parametrize("iterator_type", [iter, lambda points: (p for p in points)])
+def test_validate_locations_iterators(validator, count, iterator_type):
+    points = [[i, i + 5] for i in range(count)]
+    assert validator(iterator_type(points)) == points
+
+
+@pytest.mark.parametrize("validator", [validate_locations, validate_multi_locations])
+def test_validate_locations_empty_iterator(validator):
+    with pytest.raises(ValueError, match="Locations is empty"):
+        validator(iter([]))
+
+
 @pytest.mark.parametrize(
     "locations",
     [
@@ -91,6 +105,15 @@ def test_validate_multi_locations(locations):
     assert outcome == [[[0, 5], [1, 6], [2, 7]], [[3, 8], [4, 9]]]
 
 
+@pytest.mark.parametrize("outer_iterator", [False, True])
+def test_validate_multi_locations_iterators(outer_iterator):
+    lines = [[[0, 5], [1, 6], [2, 7]], [[3, 8], [4, 9]]]
+    locations = [iter(line) for line in lines]
+    if outer_iterator:
+        locations = iter(locations)
+    assert validate_multi_locations(locations) == lines
+
+
 @pytest.mark.parametrize(
     "locations",
     [