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bwamem3/index, bwamem3/mem: bump bwa-mem3 to 0.17.0 - #13715

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eduard-watchmaker merged 1 commit into
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bwamem3-0.17.0
Nov 13, 2026
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eduard-watchmaker merged 1 commit into
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bwamem3-0.17.0

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Bumps bwamem3/index and bwamem3/mem to bwa-mem3 0.17.0, as suggested by @nh13 on nf-core/methylseq#638. It is faster and uses less memory under --meth.

Output changes:

  • Bare --meth (bisulfite/EM-seq): scoring now defaults to --meth-scoring genomic (feat(meth)!: default EM-seq/bisulfite --meth-scoring to genomic fg-labs/bwa-mem3#756), so scores, MAPQ, NM and some placements change. The module tests record read sequences and headers rather than scores, so their snapshots only change in the version strings. ext.args = '--meth --meth-scoring collapsed' keeps the 0.16.0 output; this is now noted in the mem meta.yml.
  • --meth=taps and plain DNA alignment: unchanged.
  • Indexes: byte-identical to 0.16.0, plain and --meth, so existing indexes keep working.
  • Under -u: alternative hits are now written as XB:Z instead of XA:Z. No nf-core component passes -u.

Changes

  • bwamem3/index, bwamem3/mem: bwa-mem3 0.16.0 → 0.17.0, with new Seqera containers, conda lock files and meta.yml container entries (nf-core modules containers create).
  • bwamem3/mem meta.yml: note on the new bare --meth scoring default and how to keep the old one.
  • Module snapshots regenerated on linux/amd64; only the version strings change. The subworkflows that use these modules (fasta_index_dna, fasta_index_methylseq, fastq_align_dedup_bwamem3 and fastq_align_dna) pass against their existing snapshots, unchanged.

Generated by Claude Code

PR checklist

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
  • If you've added a new tool - have you followed the module conventions in the contribution docs
  • If necessary, include test data in your PR.
  • Remove all TODO statements.
  • Emit the versions.yml file.
  • Follow the naming conventions.
  • Follow the parameters requirements.
  • Follow the input/output options guidelines.
  • Add a resource label
  • Use BioConda and BioContainers if possible to fulfil software requirements.
  • Ensure that the test works with either Docker / Singularity. Conda CI tests can be quite flaky:
    • For modules:
      • nf-core modules test bwamem3/index --profile docker
      • nf-core modules test bwamem3/mem --profile docker
    • For subworkflows:
      • nf-core subworkflows test fasta_index_dna --profile docker
      • nf-core subworkflows test fasta_index_methylseq --profile docker
      • nf-core subworkflows test fastq_align_dedup_bwamem3 --profile docker
      • nf-core subworkflows test fastq_align_dna --profile docker

🤖 Generated with Claude Code

0.17.0 is faster and uses less memory under --meth. Bare --meth
(bisulfite/EM-seq) now defaults to --meth-scoring genomic; the mem
meta.yml notes how to keep the 0.16.0 scoring. --meth=taps and plain
DNA output, and the index, are unchanged.

Generated by Claude Code

Co-Authored-By: Claude Opus 5.5 <noreply@anthropic.com>
@eduard-watchmaker
eduard-watchmaker added this pull request to the merge queue Nov 13, 2026
Merged via the queue into master with commit 2d590fd Nov 13, 2026
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@eduard-watchmaker
eduard-watchmaker deleted the bwamem3-0.17.0 branch November 13, 2026 16:07
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